Figure 8.

Intracranial injection of aSyn PFFs induces formation of intraneuronal pathology in Snca^wt/GFP mice. Five micrograms of recombinant mouse aSyn PFFs were injected into the hippocampus of Snca^wt/wt, Snca^wt/GFP, or Snca^GFP/GFP mice. Brains were analyzed 30 d postinjection (dpi) by immunohistochemistry using anti-pSyn (81A; A) and immunofluorescence using an anti-pSyn (81A) or anti-GFP antibody (B). C, Quantification of the data in A. Plots indicate mean ± SEM. Asterisks indicate statistical significance following one-way ANOVA (brain area) with Tukey’s post hoc test; *p < 0.05, **p < 0.005. CA = cornu ammonus; DG = dentate gyrus. Scale bars: 50 µm, 10 µm (crop). Co-localization of PFF-induced pathology in other brain regions are shown in Extended Data Figures 8-1, 8-2, 8-3.