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. 2020 Jul 20;48(15):8490–8508. doi: 10.1093/nar/gkaa597

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Measuring the number of pol V foci per cell following ciprofloxacin (30 ng/mL) or trimethoprim (1 μg/ml) treatment under normal conditions or ROS-mitigating (2% DMSO) conditions in lexA(Def) cells. (A) Formation of UmuC-mKate2 foci in lexA(Def) cells (RW1286). Cells were treated for 60 min prior to imaging. Upper row: unfiltered image of an average projection showing UmuC-mKate2 foci that persist for >1 s (from left to right: ciprofloxacin, ciprofloxacin-DMSO, trimethoprim, trimethoprim-DMSO). Bottom row: merged image showing UmuC-mKate2 foci in magenta and DnaX-YPet foci in green (from left to right: ciprofloxacin, ciprofloxacin-DMSO, trimethoprim, trimethoprim-DMSO). Scale bar represents 5 μm. (B) Number of UmuC-mKate2 foci per cell of foci that persist for >1 s following ciprofloxacin treatments (30 ng/ml). Error bars represent standard error of the mean. Number of cells included in analysis: n(ciprofloxacin) = 97, n(ciprofloxacin-DMSO) = 109, n(untreated) = 87. (C) Number of UmuC-mKate2 foci per cell of foci that persist for >1 s following trimethoprim treatments (1 μg/ml). Error bars represent standard error of the mean. Number of cells included in analysis: n(trimethoprim) = 102, n(trimethoprim-DMSO) = 120, n(untreated) = 87.