Table 1.
Strains used in this study
| Strain | Relevant genotype | Parent strain | Source/technique |
|---|---|---|---|
| MG1655 | dinB+ dnaX+ recB+ lexA+ | Published (92) | |
| EAW102 | ΔrecB::KanR | MG1655 | Lambda Red recombination |
| HG356 | ΔrecB::FRT | MG1655 | EAW102 |
| DE407 | lexA3(Ind−) | DE192 | Published (48) |
| RW1568 | lexA3(Ind−) | MG1655 | Transduction of MG1655 with P1 grown on DE407 |
| EAW114 | ΔrecO::KanR | MG1655 | Lambda Red recombination |
| EAW629 | ΔrecF::KanR | MG1655 | Lambda Red recombination |
| EAW669 | ΔrecR::KanR | MG1655 | Lambda Red recombination |
| EAW671 | ΔrecF::FRT ΔrecR::KanR | EAW669 | Transduction of EAW669 with P1 grown on EAW629 |
| EAW693 | ΔrecF::FRT ΔrecR::FRT ΔrecO::KanR | EAW671 | Transduction of EAW671 with P1 grown on EAW114 |
| EAW287 | recA(E38K) sulA−::FRT | MG1655 | Published (50) |
| EAW18 | ΔdinB::KanR | MG1655 | Published (30) |
| EAW642 | dnaX-mKate2::KanR | MG1655 | Published (30) |
| EAW633 | dinB-YPet::KanR | MG1655 | Published (30) |
| EAW643 | dinB-YPet::FRT dnaX-mKate2::KanR | EAW633 | Published (30) |
| JJC5945 | dnaX-YPet::KanR | MG1655 | Published (50) |
| EAW191 | umuC-mKate2::KanR | MG1655 | Published (50) |
| EAW282 | umuC-mKate2::FRT dnaX-YPet::KanR | JJC5945 | Published (50) |
| RW120 | recA + sulA − lexA + ΔumuDC::CmR | RW118 | Published (49) |
| RW880 | ΔumuDC::CmR | MG1655 | Transduction of MG1655 with P1 grown on RW120 (49) |
| EAW13 | sulA −::KanR | MG1655 | Published (50) |
| EAW1134 | dinB-YPet::FRT dnaX-mKate2::FRT sulA−::KanR | EAW643 | Transduction of EAW643 with P1 grown on EAW13 (46) |
| DE406 | lexA(Def)::CmR | DE192 | Published (51) |
| EAW1141 | dinB-YPet::FRT dnaX-mKate2::FRT sulA−::FRT lexA51(Def)::CmR | EAW1134 | Transduction of EAW1134 with P1 grown on DE406 (51) |
| EAW1144 | dinB-YPet::FRT dnaX-mKate2::FRT sulA−::FRT lexA51(Def)::CmR ΔrecB::KanR | EAW1141 | Transduction of EAW1141 with P1 grown on EAW102 |
| RW244 | recA730 srlD300::Tn10 | MG1655 | Published (52) |
| RW1594 | dinB-YPet dnaX-mKate2 sulA −::KanRlexA51(Def)::CmR | RW1588 | Published (30) |
| RW1598 | dinB-YPet dnaX-mKate2 sulA::kanRlexA(Def)::CmRrecA(E38K) srlD300::Tn10 | RW1594 | Transduction of RW1594 with P1 grown on RW244 |
| EAW830 | dinB(D103N)-YPet | MG1655 | Published (30) |
| AR023 | recA730 sulA −::FRT dinB(D103N)-YPet | EAW287 | Transduction of EAW287 with P1 grown on EAW830 |
| EAW282 sulA− | umuC-mKate2::FRT dnaX-YPet::FRT sulA−::KanR | EAW282 | Transduction of EAW282 with P1 grown on EAW13 |
| RW1286 | umuC-mKate2::FRT dnaX-YPet::FRT sulA−::KanRlexA51(Def)::CmR | EAW282 sulA− | Transduction of EAW282 sulA− with P1 grown on DE406 |
| RW546 | recA+ sulA − lexA51(Def) ΔumuDC::CmR | RW542 | Published (93) |