Figure 9.

Characterization of the functions of other probable phosphorylation targets in Rad51-NTD and the fourth SQ motif. (A) Protein sequence alignments showing Rad51-NTD variants with serines and threonines (blue) replaced by alanines (red), as indicated. (B) Spot assay showing five-fold serial dilutions of haploid yeast strains, performed as described in Figure 2. (C, D) Total cell lysates were prepared from mitotic cells under MMS (0.02%) treatment and analyzed using immunoblots, as described in Figure 3. Protein levels were quantified and normalized to Hsp104 levels. Protein levels from WT lysates of MMS-treated cultures were a standard for comparison in (C). WT lysates without MMS treatment were used as a standard for comparison in (D).