Table 1.
Spore viability
| Tetrad class (viable: non-viable) | ||||||
|---|---|---|---|---|---|---|
| Strain | Spore viabilitya | 4:0 | 3:1 | 2:2 | 1:3 | 0:4 |
| RAD51 | 97% (n = 152) | 92% | 5% | 3% | 0% | 0% |
| rad51Δ | 0% (n = 100) | 0% | 0% | 0% | 0% | 100% |
| rad51-ΔN | 51% (n = 216) | 7% | 30% | 28% | 28% | 7% |
| rad51-ΔN sml1Δ | 83% (n = 144) | 42% | 53% | 3% | 3% | 0% |
| rad51-S2A | 99% (n = 216) | 98% | 0% | 2% | 0% | 0% |
| rad51-S12A | 100% (n = 216) | 100% | 0% | 0% | 0% | 0% |
| rad51-S30A | 100% (n = 216) | 98% | 2% | 0% | 0% | 0% |
| rad51-S2A S12A | 100% (n = 216) | 98% | 2% | 0% | 0% | 0% |
| rad51-3A | 97% (n = 216) | 94% | 2% | 2% | 0% | 2% |
| rad51-3D | 97% (n = 288) | 96% | 2% | 0% | 0% | 2% |
| dmc1Δ hed1Δ | 74% (n = 216) | 61% | 2% | 20% | 6% | 11% |
| rad51-3A dmc1Δ | No spore | — | — | — | — | — |
| rad51-3D dmc1Δ | No spore | — | — | — | — | — |
| rad51-ΔN dmc1Δ | No spore | — | — | — | — | — |
| rad51-3A dmc1Δ hed1Δ | No spore | — | — | — | — | — |
| rad51-3D dmc1Δ hed1Δ | 70% (n = 220) | 53% | 4% | 27% | 4% | 13% |
| rad51-ΔN dmc1Δ hed1Δ | No spore | — | — | — | — | — |
| mec1-kd sml1Δ dmc1Δ hed1Δ | 7% (n = 216) | 2% | 2% | 6% | 4% | 87% |
aSpore viability was analyzed after 3 days on sporulation media at 30°C. To score spore viability, only tetrads (but not dyads or triads) were dissected on YPD.