Table 2.
Spore viability of yeast strains with overexpression plasmids
| Strain | Overexpression plasmida | Spore viability |
|---|---|---|
| WT | 2μ-bsd-RAD51 | 100% (n = 144) |
| rad51Δ | 2μ-bsd-RAD51 | 98% (n = 288) |
| rad51-ΔN | 2μ-bsd-RAD51 | 80% (n = 144) |
| dmc1Δ hed1Δ | 2μ-bsd-RAD51 | 83% (n = 216) |
| dmc1Δ hed1Δ rad51-3A | 2μ-bsd-RAD51 | 85% (n = 216) |
| dmc1Δ hed1Δ rad51-ΔN | 2μ-bsd-RAD51 | 81% (n = 144) |
| WT | 2μ-bsd-rad51-3A | 47% (n = 216) |
| rad51Δ | 2μ-bsd-rad51-3A | 97% (n = 216) |
| dmc1Δ | 2μ-bsd-rad51-3A | No spore formation |
| dmc1Δ hed1Δ | 2μ-bsd-rad51-3A | 86% (n = 216) |
| dmc1Δ hed1Δ rad51-3A | 2μ-bsd-rad51-3A | 79% (n = 216) |
| WT | 2μ-bsd-rad51-ΔN | 97% (n = 144) |
| rad51Δ | 2μ-bsd-rad51-ΔN | 63% (n = 144) |
| rad51-ΔN | 2μ-bsd-rad51-ΔN | 54% (n = 144) |
| dmc1Δ hed1Δ rad51-ΔN | 2μ-bsd-rad51-ΔN | No spore formation |
| rad51Δ | mock | 0% (n = 144) |
| rad51-ΔN | mock | 38% (n = 144) |
| dmc1Δ hed1Δ | mock | 88% (n = 216) |
| dmc1Δ hed1Δ rad51-3A | mock | No spore formation |
| dmc1Δ hed1Δ rad51-ΔN | mock | No spore formation |
aYeast diploid cells were respectively transformed with the indicated 2μ overexpression vector. Resistance to blasticidin is conferred by the product of the blasticidin S deaminase (bsd) gene from Aspergillus terreus. The RAD51, rad51-3A and rad51-ΔN genes were expressed under the native RAD51 promoter.