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. 2020 Jul 3;48(15):8360–8373. doi: 10.1093/nar/gkaa558

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — RPH1 is genetically involved in the TORC1 pathway. (A) The indicated strains were spotted on YPD plates with low dose of rapamycin. Pictures were taken after grown 2 or 4 days. (B) Tet-off system derived yeast strains were spotted on plates with the indicated doses of rapamycin in the presence of doxycycline. (C) Log-phase cells were cultured under rapamycin treatment, and were isolated at the indicated times and subjected to immunoblot with different antibodies. G6PDH served as a loading control. Asterisk indicates a non-specific band. (D) The phosphorylated Rps6 levels relative to total Rps6 levels in panel C were plotted with the indicated time points and then normalized to the time 0 point of individual strain by densitometric analysis using ImageJ software. The error bars represent mean ± SD from three independent biological repeats. t-test, ^∗∗P < 0.01.