Figure 1.

Schematic of CAP-STRUCTURE-seq. (+)SHAPE sequencing library generation showing NAI treatment, nucleotide modification and purification of intact mRNA steps. Arabidopsis thaliana etiolated seedlings were treated with NAI. After extraction of total RNA, degraded mRNAs (purple) were removed, leaving intact mRNAs characterized by 5′CAP and 3′ polyA+ (dark yellow). cDNAs (orange) were obtained by reverse transcription (RT) and subjected to an established library construction. RT, reverse transcription.