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. 2020 Jul 11;48(15):8767–8781. doi: 10.1093/nar/gkaa577

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Validation of TAM functionality by a designed structure assay. (A) Cartoon representation of the protoplast transformation assay to validate the TAM functionality using a designed structure assay. GQS refers to a G-quadruplex. The miRNA156 target sites (blue comb) followed by 0 or 2 Adenines (As) and ending with a GQS. The prefixes, ‘0A’ and ‘2A’, indicate the number of Adenines. (B) In vivo RNA structures of 0A_GQS and 2A_GQS. (C) The non-cleaved mRNA abundance for the structures in (B) was measured by qRT-PCR (dark yellow bars). P-value < 0.01 by Student's t-test. The antisense target sites were used as controls (teal bars). Data are mean ± SEM from three independent biological replicates.