Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 2;48(15):8645–8662. doi: 10.1093/nar/gkaa575

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — KPAF5 is essential for viability of procyclic and bloodstream parasite forms. (A) Growth kinetics of procyclic (PF) and bloodstream (BF) parasite cultures after mock treatment and RNAi induction with tetracycline. Data from three independent experiments are shown as mean ± s.d. (B) Northern blotting analysis of KPAF4 mRNA downregulation by inducible RNAi in BF. (C) Real-time RT-PCR analysis of RNAi-targeted KPAF5 mRNA, mitochondrial rRNAs and mRNAs. RNA levels were normalized to β-tubulin mRNA. RNAi was induced for 72 h. Error bars show the standard deviation from at least three biological replicates. The thick line at ‘1’ reflects no change in relative abundance; bars above or below represent an increase or decrease, respectively. P, pre-edited mRNA; E, edited mRNA.