Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 2;48(15):8645–8662. doi: 10.1093/nar/gkaa575

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Stage-specific effects of KPAF4 and KPAF5 knockdowns on pan-edited and moderately-edited mRNAs. (A) Northern blotting of pre-edited (Pre-E) and fully-edited (E) RPS12 and A6 mRNA variants. Total RNA was separated on a 5% polyacrylamide/8M urea gel and sequentially hybridized with radiolabeled DNA probes. Parent: Lister 427 29-13 strain; (dT), total RNA from parental cell line was hybridized with 20-mer oligo(dT) and treated with RNase H to locate non-adenylated molecules. Zero time point: mock-induced RNAi cell line. Cytosolic 5.8S rRNA was used as loading control. (B) Northern blotting of moderately-edited Cyb mRNA. Total RNA was separated on a 1.7% agarose/formaldehyde gel and hybridized with oligonucleotide probes for pre-edited and fully-edited sequences. Loading control: cytosolic 18S rRNA.