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. 2020 Jul 7;48(15):8782–8795. doi: 10.1093/nar/gkaa578

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Substrate preference of rnGLD-2. (A) Time-course NTP consumption of rnGLD-2 with A₁₅. Error bars indicate s.d. (n = 3). (B) Time-course ATP consumption of rnGLD-2 with various 15-mer RNA oligos. (C) NTP consumption of rnGLD-2 with various 15-mer RNA oligos. (D) Nucleotidyltransferase assays showing the preference of rnGLD-2 on various substrates and different nucleotides. 400 nM rnGLD-2, 500 nM 5′ biotinylated 15-mer RNA substrates with various sequences, and 500 μM ATP/UTP/GTP/CTP were used.