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. 2020 Jul 7;48(15):8782–8795. doi: 10.1093/nar/gkaa578

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Similarity between rnGLD-2 and TUT7_CM. (A) NTase activity of rnGLD-2 on different miRNA substrates. A total of 400 nM rnGLD-2, 500 nM various 5′-biotinylated miRNA and 500 μM ATP/UTP/GTP/CTP were used. (B) Overall structure comparison between rnGLD-2 and TUT7_CM in complex with UMPNPP/U₂ (upper, PDB code: 5w0n) and with UTP/dsRNA (lower, PDB code: 5w0o). (C) Comparison between rnGLD-2 and TUT7_CM at UTP binding site. Residues of TUT7_CM (PDB code: 5w0o) involved in binding UTP is superimposed with corresponding residues of rnGLD-2. (D) Uridylation activity of rnGLD-2 with mutations regarding the residues potentially involved in UTP coordination. (E) Comparison between rnGLD-2 and TUT7_CM at substrate binding site. Residues of TUT7_CM (PDB code: 5w0n) involved in binding the U₂ substrate is superimposed with corresponding residues of rnGLD-2. (F) Adenylation and uridylation activity of rnGLD-2 with mutations regarding the residues potentially involved in substrate binding.