Figure 4.
The integrity of NC2 heterodimer is a prerequisite for NC2 to bind to cat-3 locus and to activate its expression. (A, B) ChIP assays showing the binding levels of NC2α (A) and NC2β (B) at cat-3 locus in Nc2αKO, Nc2βKO and wild-type strains. Short black lines (primer pairs 3–7) under the schematic diagram of cat-3 gene represent the regions detected by ChIP-qPCR. TSS, transcription start site; ORF, open reading frame. Error bars show s.d. (n = 3). Significance was assessed by using a two-tailed t-test. **P < 0.01 and ***P < 0.001 versus WT. (C, D) Immunoprecipitation assays showing the interaction between Myc-NC2α or Myc-NC2β and endogenous NC2β or NC2α protein, respectively.