Figure 7.

Larp4 KO compromised the expression of NFκB target genes. (A, B) The relative mRNA levels of steady-state Il2 and Ifnγ in resting and activated CD4⁺ T cells derived from the WT and Larp4 KO mice. Gapdh was used the internal control. (C–H) Analysis of cytokine secreted by resting and activated CD4⁺ T cells derived from the WT and Larp4 KO mice. The level of each cytokine in the culture supernatant was determined by the LEGENDplex™ Cytometric Bead Assay (BioLegend). The concentrations of IFNγ (C), IL2 (D), TNFα (E), IL4 (F), IL5 (G) and IL6 (H) were determined. (I) The stability of Il2 and Ifnγ mRNA transcripts, as measured by RT-qPCR following FLV treatment for the indicated time, in activated CD4⁺ T cells obtained from WT and Larp4 KO mice. Gapdh was used the internal controls.R and A represent resting and activated CD4⁺ T cells, respectively. The error bars represent ± SEM based on three biological replicates (*P < 0.05).