Figure 3.

Proportions of pre-edited, partially edited, and fully edited CYb, COIII, and A6 mRNA in procyclic and bloodstream cells. (A) Schematic for the placement of CYb, COIII and A6 high throughput sequencing (HTS) primer pairs. The primers are represented by red arrows. The never-edited regions of the mRNAs are indicated in black; the pre-edited regions are indicated in gray; the edited regions of the mRNAs are indicated in yellow. (B) Pie charts illustrating the breakdown for the percentage of CYb, COIII and A6 mRNAs by editing status in procyclic (PCF) 29–13 and bloodstream cell lines 427, 90–13, SM-1 and SM-2 as determined by HTS/TREAT analysis. Red arrows denote samples containing fully edited mRNA reads. Note that ‘fully’ edited reads for A6 and COIII refer to those reads that are canonically fully edited up to the end of the forward primer.