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. 2020 Aug 1;48(15):8704–8723. doi: 10.1093/nar/gkaa641

Figure 6.

Figure 6.

Statistical and bioinformatic analyses of COIII mRNA editing intermediates at the final prominent EPS in PCF and BSF SM-1 cells. (A) Schematic illustrating the positions of exacerbated pause sites (EPSs) in BSF COIII mRNA in relation to the position of gRNAs. Symbols as in Figure 5. The red diamond represents the final prominent EPS at ES37 in this cell line. (B) Sequence alignments of the 10 most abundant junction sequences at ES37 in COIII mRNA of PCF and BSF SM-1 cells. Symbols and shading as in Figure 5. (C) Schematic of terminator guide RNAs found in PCF (gRNAs A–C) and BSF (gRNAs D–E) annealed to COIII mRNA with the non-productive consensus junction sequence at ES37. The ratio for the counts for the canonical gRNAs that direct the editing past ES37 and the terminator gRNAs are shown as determined by previously published gRNA reads (61,62).