Table 1.
nEMOTE detected cleavage sites in genes involved in protein synthesis
| Gene | Gene number | Gene descriptiona | MazF motif and localizationb | GO categoryc |
|---|---|---|---|---|
| rlmB | SAOUHSC_00513 | 23S rRNA (guanosine(2251)-2′-O)-methyltransferase RlmB | [290/UACUUA] | RNA methylation [GO:0001510] |
| rplK | SAOUHSC_00518 | 50S ribosomal protein L11 | [233/UACUUA] | Structural constituent of ribosome [GO:0003735] |
| rplA | SAOUHSC_00519 | 50S ribosomal protein L1 | []104/UACCUA | Structural constituent of ribosome [GO:0003735] |
| rpsG | SAOUHSC_00528 | 30S ribosomal protein S7 | 5/UACAUU[] | Structural constituent of ribosome [GO:0003735] |
| SAOUHSC_00580 | SAOUHSC_00580 | Hypothetical protein | [9/UACAUU] | n/a |
| prfB | SAOUHSC_00771 | Peptide chain release factor 2 | [492/UACAUU] | n/a |
| trpS | SAOUHSC_00933 | Tryptophanyl-tRNA synthetase | [372/AACAUA] | tRNA aminoacylation for protein Translation [GO:0006418] |
| prfC | SAOUHSC_00956 | Peptide chain release factor 3 | [590/UACAUU] | Regulation of translational Termination [GO:0006449] |
| pheS | SAOUHSC_01092 | Phenylalanyl-tRNA synthetase subunit alpha | [100/UACUUA] | Phenylalanyl-tRNA aminoacylation [GO:0006432] |
| ileS | SAOUHSC_01159 | Isoleucyl-tRNA synthetase | [188/UACAUA] | n/a |
| rpsP | SAOUHSC_01208 | 30S ribosomal protein S16 | [114/UACUUA] | Ribosome [GO:0005840] |
| Gid | SAOUHSC_01223 | tRNA (uracil-5-)-methyltransferase Gid | [526/UACUUA] | tRNA wobble uridine modification [GO:0002098] |
| infB | SAOUHSC_01246 | Translation initiation factor IF-2 | [1257/UACUUA] | Translational initiation [GO:0006413] |
| rpsA | SAOUHSC_01493 | 30S ribosomal protein S1 | [253/UACAUC] | Structural constituent of ribosome [GO:0003735] |
| Efp | SAOUHSC_01625 | Elongation factor P | [310/UACUUA] | translation elongation factor activity [GO:0003746] |
| aspS | SAOUHSC_01737 | Aspartyl-tRNA synthetase | [1362/UACAUC] | Aspartyl-tRNA aminoacylation [GO:0006422] |
| queA | SAOUHSC_01749 | S-adenosylmethionine:tRNA ribosyltransferase-isomerase | [457/UACAUC] | n/a |
| valS | SAOUHSC_01767 | valyl-tRNA synthetase | [229/UACUUA] | Valyl-tRNA aminoacylation [GO:0006438] |
| rpsD | SAOUHSC_01829 | 30S ribosomal protein S4 | [175/UACUUA] | Structural constituent of ribosome [GO:0003735] |
| leuS | SAOUHSC_01875 | Leucyl-tRNA synthetase | [575/UACUUA] | Aminoacyl-tRNA editing activity [GO:0002161] |
| SAOUHSC_02248 | SAOUHSC_02248 | Hypothetical protein | [417/UACAUC] | n/a |
| SAOUHSC_02297 | SAOUHSC_02297 | S1 RNA-binding domain-containing protein | [1662/UACAUC] | Structural constituent of ribosome [GO:0003735] |
| rpsI | SAOUHSC_02477 | 30S ribosomal protein S9 | [314/UACUUA] | Structural constituent of ribosome [GO:0003735] |
| rpsE | SAOUHSC_02494 | 30S ribosomal protein S5 | 2/UACAUG[] | Structural constituent of ribosome [GO:0003735] |
| rplX | SAOUHSC_02501 | 50S ribosomal protein L24 | [2/UGCAUA] | Structural constituent of ribosome [GO:0003735] |
| rplP | SAOUHSC_02505 | 50S ribosomal protein L16 | [169/UACAUG] | Structural constituent of ribosome [GO:0003735] |
| rpsC | SAOUHSC_02506 | 30S ribosomal protein S3 | [570/UACUUA] | Structural constituent of ribosome [GO:0003735] |
| SAOUHSC_02512a | SAOUHSC_02512a | 30S ribosomal protein S10 | [145/UACACA] | n/a |
| SAOUHSC_02519 | SAOUHSC_02519 | Hypothetical protein | [406/UACAUA] | n/a |
| SAOUHSC_02827 | SAOUHSC_02827 | Hypothetical protein | [167/UACUUA] | n/a |
| rpmH | SAOUHSC_03055 | 50S ribosomal protein L34 | [12/UACUUA] | Structural constituent of ribosome [GO:0003735] |
| rpsF | SAOUHSC_00348 | 30S ribosomal protein S6 | [6/AACAUA] [22/UACAUC] | Structural constituent of ribosome [GO:0003735] |
| fusA | SAOUHSC_00529 | Elongation factor G | 2/UACAUG[] [829/UACUUA] | GTPase activity [GO:0003924] |
| SAOUHSC_01091 | SAOUHSC_01091 | SpoU rRNA Methylase family protein | [665/UACCUA] []186/UACUUA | n/a |
| Tsf | SAOUHSC_01234 | Elongation factor Ts | [466/UACUUA] [548/UGCAUA] | Translational elongation [GO:0006414] |
| asnC | SAOUHSC_01471 | Asparaginyl-tRNA synthetase | [789/UACAUC] [559/UACUUA] | Asparaginyl-tRNA aminoacylation [GO:0006421] |
| queG | SAOUHSC_01989 | Hypothetical protein | [867/AACAUA] [839/UACGUA] | Epoxyqueuosine reductase activity [GO:0052693] |
| infA | SAOUHSC_02489 | Translation initiation factor IF-1 | [201/UACUUA] [130/UACAUU] | Translation initiation factor activity [GO:0003743] |
| rplN | SAOUHSC_02502 | 50S ribosomal protein L14 | []15/UACAAA [226/UACAUC] | Structural constituent of ribosome [GO:0003735] |
| cysS | SAOUHSC_00511 | Cysteinyl-tRNA synthetase | [540/UACAUU] [665/UACAUG] [1017/GACAUA] | Cysteinyl-tRNA aminoacylation [GO:0006423] |
| rpsL | SAOUHSC_00527 | 30S ribosomal protein S12 | [185/UACGUA] [235/UACAUC] [251/GACAUA] | Structural constituent of ribosome [GO:0003735] |
| rpsS | SAOUHSC_02508 | 30S ribosomal protein S19 | [234/UACAUU] [137/GACAUA] 25/CACAUA[] | Structural constituent of ribosome [GO:0003735] |
| Tuf | SAOUHSC_00530 | Elongation factor Tu | [591/UACUUA] [595/UACAUU] [743/UACAUG] [928/UACGUA] | Translational elongation [GO:0006414] |
| rplC | SAOUHSC_02512 | 50S ribosomal protein L3 | [324/UACUUU] | Structural constituent of ribosome [GO:0003735] |
| tadA | SAOUHSC_00541 | Hypothetical protein | []8/UACAUA | n/a |
| trnaT | SAOUHSC_T00055 | tRNA-Thr | 7/UACAUA[] | n/a |
| hemA | SAOUHSC_01776 | Glutamyl-tRNA reductase | [455/CACAUA] | Glutamyl-tRNA reductase activity [GO:0008883] |
| SAOUHSC_00526 | SAOUHSC_00526 | 50S ribosomal protein L7Ae-like protein | [90/UACAUC] | Ribosome [GO:0005840] |
| ychF | SAOUHSC_00346 | GTP-dependent nucleic acid-binding protein EngD | [152/UACUUA] [846/AACAUA] | Ribosome binding [GO:0043022] |
| hflX | SAOUHSC_01283 | Hypothetical protein HflX | [422/UACCUA] | Ribosome binding [GO:0043022] |
aGene product as annotated in Aureowiki for NCTC8325 genome
bBrackets represent the ORF; number inside brackets indicate The MazF cleavage position relative to the ATG codon; while brackets before or after the cleavage position indicates cleavage outside the ORF, downstream or upstream respectively.
cComplete gene ontology enrichment analysis in Supplementary Table S6.