Table 4.
MazF cleavage on potential MazEF TAS regulatory circuit
| Gene | Gene number | Gene descriptiona | MazF motif and localizationb | Detection method |
|---|---|---|---|---|
| sigB | SAOUHSC_02298 | RNA polymerase sigma factor SigB | [317/UACAUG] | Predicted, probe |
| rsbW | SAOUHSC_02299 | Serine–protein kinase RsbW | [135/UACAUA] | Predicted, probe |
| rsbV | SAOUHSC_02300 | STAS domain-containing protein | [232/UACAUU] | Probe, nEMOTE |
| rsbU | SAOUHSC_02301 | Phosphatase RsbU | No motif | – |
| mazF | SAOUHSC_02303 | Hypothetical protein | [277/UACUUA] | nEMOTE |
| mazE | SAOUHSC_02304 | Hypothetical protein | No motif | – |
| clpC | SAOUHSC_00505 | Endopeptidase | [293/UACAUC] | Probe, nEMOTE |
| clpP | SAOUHSC_00790 | ATP-dependent Clp protease proteolytic subunit | [187/UACAUU] | Predicted, No experimental detection |
| spxA | SAOUHSC_00934 | Transcriptional regulator Spx | [341/UACCUA] | nEMOTE |
| trfA | SAOUHSC_00935 | Adaptor protein MecA | [191/UACAUG] | Predicted, no experimental detection |
| sarA | SAOUHSC_00620 | Accessory regulator A | [124/UACAUC] | Predicted, no experimental detection |
| sarS | SAOUHSC_00070 | Accessory regulator-like protein | [273/UACUUA] | Probe, nEMOTE |
aGene product as annotated in Aureowiki for NCTC8325 genome
bBrackets represent the ORF; number inside brackets indicate the MazF cleavage position relative to the ATG codon; brackets before or after the cleavage position indicates cleavage outside the ORF, downstream or upstream respectively.