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Iron content (mol Fe mol−1 ribosome) of purified ribosomes. E. coli were grown aerobically or anaerobically at 1 mM Fe2+ or ambient Fe2+ (6–9 μM, no Fe added), and purified in buffers containing either 3 mM Mg2+ (black circles) or 1 mM Fe2+ (white circles). Error bars represent the SEM (n = 3).
