FIG 2.

Doubling times in YPD medium at 30°C. Each data point is representative of the average doubling time (in minutes) (n = 8) of the parental (SN148) strain (red square) or a constructed strain (circles, CRISPR-Cas9-free method; diamonds, CRISPR-Cas9-dependent method), with error bars indicating standard deviations. The average doubling time of all constructed strains (57 strains) is represented by the orange horizontal line (with orange shading indicating standard deviations). Strains displaying at least one genomic change are shown in yellow, while black indicates strains that are free of transformation-acquired genomic changes.