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. 2020 Jul 8;11(9):2398–2405. doi: 10.1111/1759-7714.13534

Figure 4.

Figure 4

The interaction between TMPO‐AS1 and miR‐498. (a) The interaction between TMPO‐AS1 and miR‐498 was predicted by starBase. The red color represents the mutated sites. (b and c) The luciferase activity was analyzed in EC109 and KYSE70 cells transfected with TMPO‐AS1 WT or TMPO‐AS1 MUT and miR‐498 or miR‐NC. EC109: Inline graphic, miR‐NC; Inline graphic, miR‐498; and Kyse70: Inline graphic, miR‐NC; Inline graphic, miR‐498. (d and e) RIP assay was used to verify the interaction between TMPO‐AS1 and miR‐498. EC109: Inline graphic, anti‐lgG; Inline graphic, anti‐ago2; Inline graphic, Input; and Kyse70: Inline graphic, anti‐lgG; Inline graphic, anti‐ago2; Inline graphic, Input. (f) TMPO‐AS1 expression was detected in EC109 and KYSE70 cells transfected with si‐NC or si‐TMPO‐AS1. Inline graphic, si‐NC; Inline graphic, si‐TMPO‐AS1. (g) MiR‐498 level was investigated in EC109 and KYSE70 cells transfected with si‐NC, si‐TMPO‐AS1, vector, or TMPO‐AS1, respectively. *P < 0.05. Inline graphic, si‐NC; Inline graphic, si‐TMPO‐AS1; Inline graphic, vector; Inline graphic, TMPO‐AS1.