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. 2020 Aug 3;11(9):2630–2638. doi: 10.1111/1759-7714.13593

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© 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of mAb NJ001 on its specific antigen in SPC‐A1 cells. (a) Subcellular localization of NJ001 specific antigen was analyzed using immunoelectron microscope. Dots represent NJ001 specific antigens. (b) Western blots confirmed NJ001 specific antigen expression in cytoplasm, cell nucleus and cytomembrane of SPC‐A1. (c) The nuclear protein of control SPC‐A1 and SPC‐A1 treated with NJ001 were immunoprecipitated with an anti‐FOXP1 antibody or anti‐rabbit IgG, as control. The expression of NJ001 specific antigen in the immune complexes was determined by western blot using mAb NJ001.