Figure 1.
UCA1 is a direct target candidate of miR‐138/193. (a) The potential miRNAs targeting UCA1 in the region (1395–1433 bp) using bioinformatic analysis. (b) MiR‐138 and miR‐193 mimics were transfected into lung cancer cell lines (A549, H1299, and H1650). The RNA levels of UCA1 (
) NC, (
) miR‐138, and (
) miR‐193 and miRNAs (
) NC, (
) miR‐138, and (
) miR‐193 were tested using real‐time PCR. (c) The antagomirs of miR‐138 or miR‐193 were transfected into lung cancer cells and the corresponding RNA level was examined (
) NC, (
) anti‐miR‐138, and (
) anti‐miR‐193; and (
) NC, (
) anti‐miR‐138, and (
) anti‐miR‐193. (d) Construction of wild‐ and mutant‐type binding sites between UCA1 and miR‐138 or miR‐193 in luciferase reporter vector was conducted. A549, H1299 and H1650 cells were cotransfected with UCA1 and wild‐type or mutant miR‐138 (
) NC, (
) UCA1‐wt, and (
) UCA1‐mut or miR‐193 (
) NC, (
) UCA1‐wt, and (
) UCA1‐mut reporter vector, followed by the measurement of luciferase activities. *P < 0.05.