Figure 2.
In vitro effects of CEACAM6 expression on DDP resistance. (a) Lentiviral vector‐meditated siRNA to knock down CEACAM6 expression in A549/DDP cells with or without miRNA inhibitors; *P < 0.05 vs. A549, **P < 0.05 vs. A549‐siCEACAM6. (b) CEACAM6 overexpression vector was transfected into A549 cells with or without miRNA mimics; *P < 0.05 vs. A549/DDP, **P < 0.05 vs. A549/DDP‐CEACAM6. (c) Cell proliferation (
) Si NC (
) SiCEACAM6* (
) SiCEACAM6+ miR146a inhibitor ** (
) SiCEACAM6+ miR26a inhibitor ** (
) Vector (
) CEACAM6* (
) CEACAM6+ miR146a mimic ** (
) CEACAM6+ miR26a mimic ** (d) the IC50 values of DDP were analyzed using a cell counting kit‐8 (CCK‐8) assay (
) Si NC (IC50 = 29.08) (
) SiCEACAM6 (IC50=6.212) * (
) SiCEACAM6+ miR146a inhibitor (IC50 = 22.65) ** (
) SiCEACAM6+ miR26a inhibitor (IC50 = 20.43) ** (
) Vector (IC50 = 5.932) (
) CEACAM6 (IC50 = 20.26) * (
) CEACAM6+ miR146a mimic (IC50 = 8.685) ** (
) CEACAM6+ miR26a mimic (IC50 = 10.21) ** . (e) Cell migration and invasion were determined by transwell and wound healing assays (
) migration (
) invasion (
) migration (
) invasion.