Figure 1.

Survey of Recently Published Studies on Pulmonary Small Interfering RNA (siRNA) or mRNA Delivery in Animals.

Studies published between 2015 and early 2020 that reported siRNA or mRNA transfection following pulmonary delivery in animals were surveyed using an article search on PubMed with the search terms ‘siRNA’ or ‘mRNA’, ‘pulmonary delivery’, ‘intratracheal’, ‘inhalation’, ‘nebulization’, and with the filters ‘last 5 years’ (publication date) and ‘other animals’ (species) applied (N = 53). The studies are classified by (A) type of delivery vector; (B) disease model used; (C) administration method used in animal studies; and (D) RNA type. The classification was done independently by Y.Q. and M.C. and was cross-validated by J.K.W.L. Abbreviation: LIRI, lung ischemia–reperfusion injury.