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. 2020 Jul 30;39(36):5888–5901. doi: 10.1038/s41388-020-01408-7

Fig. 4. PI induces MLL dysfunction through H2Bub depletion.

Fig. 4

a The indicated cells were treated with IRAK1/4 inhibitor for 48 h. Nucleoplasm and chromatin-bound fractions were purified and immunoblots of the indicated antibodies were analyzed. b Immunoblots of H2Bub treated with bortezomib (10 nM) for 8 h in the indicated cells. c SEM cells were infected by the indicated lentiviral vectors. ChIP analyses at the promoter and coding region of the CDKN1B locus in the indicated cells were detected. d SEM cells were infected by the indicated lentiviral vectors. Immunoblots of WCL and chromatin-bound proteins were detected. e Immunoblots of whole-cell lysates (WCL) and chromatin-bound proteins in the indicated SEM or RS4;11 cells. f SEM cells subjected to anti-H2Bub immunoprecipitation (IP) and analyzed with the indicated antibodies. g SEM cells of the indicated knockdown were subjected to anti-H2Bub IP and analyzed with the indicated antibodies.