Table 6.
Change of DNA methylation, ginsenosides yields and plant ontogeny characteristics after different cold exposure durations
| Cold exposure duration | Rhizomes |
Early leaves |
Final roots |
Plant ontogeny characteristics |
|---|---|---|---|---|
| 5-methylcytosine (%) | 5-methylcytosine (%) | Ginsenosides (mg) | ||
| No cold exposure (CE-0) | 17.16 ± 0.39 | -- | -- | No plants sprouted |
| Nov (CE-1) | 19.77 ± 0.44 | 17.88 ± 0.36 | -- | A very few plants sprouted but died rapidly |
| Nov to Dec (CE-2) | 23.25 ± 0.71 | 17.09 ± 0.47 | 90.79 ± 4.09 | Partial plants sprouted but prematured early |
| Nov to Jan (CE-3) | 23.38 ± 0.68 | 15.78 ± 0.22 | 168.95 ± 9.79 | Most plant sprouted but prematured early |
| Nov to Feb (CE-4) | 23.15 ± 0.77 | 14.27 ± 0.42 | 188.75 ± 11.41 | Most plant sprouted but prematured early |
| Natural temperature (CE-N) | 17.34 ± 0.56 | 12.63 ± 0.31 | 311.38 ± 12.33 | Normal sprouted, flowered and fruited |
Rhizomes are sampled at the end of Oct (CE-0), Nov (CE-1), Dec (CE-2), Jan (CE-3), Feb (CE-4), Mar (CE-N); early leaves are sampled at the fifth day after seedling sprouted; final roots are sampled at the final plant harvest day on 8 October 2017.