Table 3.

Clinico-pathological and molecular features in the recurrent ASCC group

	HPV-positive	HPV-negative	p value (Χ2 test)	HPV16 viral load (log10 transformed)	p value (Mann-Whitney U test)
Age (years), median (range)	65.5 years (30.8–81)	71 years (61–77.2)		1.56
Sex
Male	1 (20%)	4 (80%)		1.19
Female	11 (92%)	1 (8%)	p = 0.003	1.61	p = 1.0
Tumor classification
T1 + T2	1 (25%)	3 (75%)		1.11
T3 + T4	11 (85%)	2 (15%)	p = 0.02	1.61	p = 1.0
Lymph node involvement
Negative	6 (60%)	4 (40%)		2.17
Positive	6 (86%)	1 (14%)	p = 0.25	1.23	p = 0.12
Smoking statusa
Nonsmoker	6 (86%)	1 (14%)		1.61
Ever smoker	4 (57%)	3 (43%)	p = 0.23	2.67	p = 1.0
p16 expression
Negative	2 (33%)	4 (67%)		2.5
Positive	10 (91%)	1 (9%)	p = 0.01	1.55	p = 0.55
p53 staining pattern
Negative	2 (100%)	0		1.67
Scattered	8 (80%)	2 (20%)		1.43
Diffuse	2 (40%)	3 (60%)	p = 0.17 (negative vs. scattered vs. diffuse)	2.22	p = 0.82 (Kruskal-Wallis test) (negative vs. scattered vs. diffuse)
Smoking history (pack-yearsb), median (N)	20.74 pack-years (4)	6.7 pack-years (2)			p = 0.48

^aData on smoking were available for only 27 cases, and data on smoking duration and number of cigarettes smoked were available for 13/16 smokers

^bPack-year is calculated by multiplying the number of packs of cigarettes smoked per day by the number of years the person has smoked