Table 2.
Histone acetylation/methylation/phosphory marks in CRC
| Modification and sites | Method | Impaired function | References |
|---|---|---|---|
| Histone acetylation marks | |||
| Global H3ac | CHIP, WB | Hyperacetylation (CRC tissues) | [39] |
| Global H4ac | IHC | Hypoacetylayion (CRC cell lines) induced by CPERT | [40] |
| H3K9ac | IHC | Hypoacetylation (CRC liver metastasis) | [41] |
| H3K18ac | IHC | Hypoactylation (CRC cell lines) | [42] |
| H3K27ac | MS,WB | Hyperacetylation (CRC tissues) | [43] |
| H3K56ac |
WB, CHIP RT-qPCR |
Hypoacetylation (CRC cell lines) through RAS-PI3K signal pathway. | [44] |
| H4K12ac | IHC | Hypoacetylation (CRC cell lines) | [42] |
| H4K16ac |
LC-ES/MS IHC |
Hypoacetylation (CRC cell lines, CRC primary tumors) | [45, 46] |
| Histone methylation marks | |||
| H3K4me2 | CHIP, WB | Hypermethylation (CRC tissues) | [39] |
| H3K4me3 | IHC | Hypomethylation (CRC tissues) | [46] |
| H3K9me2 | IHC, WB | Hypermethylation (CRC cells line, CRC liver metastasis) | [41, 47] |
| H3K27me2 | IHC | Hypermethylation (CRC tissues) | [48] |
| H3K27me3 | IHC | Hypermethylation (CRC tissues) | [49] |
| H3K36me2 | IHC | Hypomethylation (CRC liver metastasis) | [48] |
| H3K79me2 | IHC | Hypermethylation (Patient with CRC) | [50] |
| H4K20me2 | LC-ES/MS | Hypomethylation (CRC cell lines) | [45] |
| H4K20me3 | CHIP, PCR | Hypomethylation (CRC patient’s plasma) | [51] |
| Histone phosphorylation marks | |||
| H3S10ph | IHC | Hypophosphorylation (CRC cell lines) | [52] |
| H2AX | IHC | Hyperphosphorylation (CRC patients) | [53] |