Fig. 4.

Anti–T. gondii activity of GRh2 in BALB/c mice. (A) Treatment effects on the survival of T. gondii–infected BALB/c mice. (B) QC-PCR analysis for determining T. gondii abundance in the brain. (C) Tachyzoites inhibition rate in the peritoneal cavity. (D) Immunofluorescence of T. gondii–infected PECs. The T. gondii was indicated in green, whereas the DAPI staining (blue) indicated the location and size of nuclei. Three independent experiments were conducted in triplicate, and data were expressed as the mean ± standard deviation. ^∗p < 0.05 and ^∗∗p < 0.01 vs. CN group. QC-PCR, quantitative competitive PCR; SD-Na, sulfadiazine sodium; gDNA, genomic DNA.