Fig. 6.

The effects of GRh2 on inflammatory mediator production and neuronal morphology after T. gondii infection. Western blot analysis was completed to analyze iNOS, IFN-γ, and TNF-α expression in (A) BV2 cells and (B) mouse brain tissues. (C) The nitrite concentration was determined for the BV2 cells culture supernatants or mice serum based on the ELISA. (D) and (E) Neuronal morphology and density in the cortex and hippocampus by Nissl staining. Three independent experiments were conducted in triplicate, and data were expressed as the mean ± SD. ^##p < 0.01 vs. N group; ^∗∗p < 0.01 vs. CN group. IFN-γ, Interferon-γ; iNOS, inducible nitric oxide synthase; TNF-α, tumor necrosis factor α; ELISA, enzyme-linked immunosorbent assay.