Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 3;10:14559. doi: 10.1038/s41598-020-71536-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Characterization of Sbsn^–/– mice. (a) DNA sequence. The red arrow indicates the mutation location. (b) Whole-mount in situ hybridization of E16.5. In WT mice, SBSN mRNA was markedly expressed in the dorsal skin, tail skin, and oral epithelium, but it was not seen in KO mice. (c) Western blotting. The samples were RIPA buffer extracts from the epidermis. SBSN was completely knocked out in Sbsn^–/– mice. The expressions of LOR, IVL, and FLG were retained. The blots of SBSN and β-actin were cropped from one membrane. LOR, FLG, and IVL were cropped and reprobed from another membrane. (d) Immunofluorescence staining in footpad skin. Nuclei were counterstained with DAPI. SBSN (green) is observed in the upper epidermis and stratum corneum in WT, but not in Sbsn^–/– mice. The lines illustrate the basal membrane of epidermis.