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. 2020 Sep 3;10:14559. doi: 10.1038/s41598-020-71536-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transcutaneous immunization to protein antigen in Sbsn^–/– mice. (a) OVA-specific immunoglobulins. TS, tape stripping. Trans-cutaneous immunization response was performed in mice. Any statistically differents were observed. (b) CHS response to DNFB and OX. Sbsn^–/– mice show rather lower ear swelling responses to DNFB and oxazolone than did WT mice. (c) Frequency Th2 cells induced by DNFB. The frequency of IL-4⁺CD4⁺ T cells is significantly lower in Sbsn^–/– mice than in WT mice.