Figure 5.

CHS responses to nickel in nickel-loaded mice. (a) Time course of CHS to nickel in nickel-loaded mice. Mice were fed with 100 ppm nickel (1.7 mM NiSO₄) or normal water 4 weeks before, and they were sensitized and elicited as mentioned. (b) Ear swelling responses in nickel- or water-loaded mice sensitized and challenged with nickel. Nickel loading decreased CHS response in WT mice. Sbsn^–/– mice showed augmented ear swelling by nickel loading. (c) Frequency of CD4⁺CD25⁺ T cells with or without nickel loading. While drinking of low-dose nickel-containing water increases CD4⁺CD25⁺ T cells in WT, it unaffected their frequency in Sbsn^–/– mice.