Table 1.
Overview of the 13 ATIs from wheat quantitated by targeted LC–MS/MS.
| UniProtKB accession | UniProtKB name | Abbreviation | Amino acids | Number of theoretical peptidesa | Number of identified peptidesb |
|---|---|---|---|---|---|
| P01083 | Alpha-Amylase Inhibitor 0.28 | 0.28 | 153 | 9 | 7 |
| P01085 | Alpha-Amylase Inhibitor 0.19 | 0.19 | 124 | 7 | 7 |
| P01084 | Alpha-Amylase Inhibitor 0.53 | 0.53 | 124 | 7 | 6 |
| P16850 | Alpha-Amylase/Trypsin Inhibitor CM1 | CM1 | 145 | 5 | 3 |
| P16851 | Alpha-Amylase/Trypsin Inhibitor CM2 | CM2 | 145 | 4 | 2 |
| P17314 | Alpha-Amylase/Trypsin Inhibitor CM3 | CM3 | 168 | 9 | 5 |
| P16159 | Alpha-Amylase/Trypsin Inhibitor CM16 | CM16 | 143 | 5 | 4 |
| Q41540 | CM17 Protein | CM17 | 143 | 4 | 4 |
| P16347 | Endogenous Alpha-Amylase/Subtilisin Inhibitor | WASI | 180 | 8 | 6 |
| Q43723 | Trypsin/alpha-Amylase Inhibitor CMX1/CMX3 | CMX1/3 | 121 | 5 | 1 |
| Q43691 | Trypsin/alpha-Amylase Inhibitor CMX2 | CMX2 | 121 | 5 | 1 |
| P83207 | Chymotrypsin Inhibitor | WCI | 119 | 3 | 3 |
| –c | Wheat Trypsin Inhibitor | WTI | 137 | 7 | 3 |
aDerived from in silico tryptic digestion with a minimal number of eight and a maximal number of 26 amino acids.
bIdentified by at least five transitions at the same retention time. For amino acid sequences of all identified peptides see Supplementary Table S1.
cSequence according to Altenbach et al.19; similar to UniProtKB number A0A1D5UB33 (AAI domain-containing wheat protein), 70% identity.