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. 2020 Sep 3;10:14589. doi: 10.1038/s41598-020-71249-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rec-Lcn2 and T3 did not alter the viability of 3T3-L1 cells at the tested dose. Mature 3T3-L1 cells were treated with different concentration of rec-Lcn2 and T3 separately for 24–76 h. The cell viability was estimated using Prestoblue Cell Viability Reagent according to manufacturer’s protocol. Absorbance was recorded at 415 nm using an HT Synergy microplate reader. Values for percent cell viability were normalized to the cell viability of cells with no exposure to rec-Lcn2 (vehicle control). Data shown are the average of two individual experiments. Each experiment was carried in triplicate. P value < 0.05 considered as statistically significant. Two-way ANOVA with Bonferroni post-Hoc test was used to calculate the statistical significance.