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. 2020 Sep 3;10:14589. doi: 10.1038/s41598-020-71249-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rec-Lcn2 increases mitochondrial mass. Mature 3T3-L1 cells were cultured and treated with various concentration of rec-Lcn2 (0–50 ng/ml). After 24hrs, the cells were treated with MitoTracker reagents as described under material and methods. Photographs (A) and data (B) are representative of two individual experiments; experiment was carried out in triplicate. P value < 0.05 considered as statistically significant. One-way ANOVA with Newmann-Keuls post-hoc test used to calculate the statistical significance.