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. 2020 Sep 3;10:14589. doi: 10.1038/s41598-020-71249-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lcn2 deficiency leads to development of age-related weight gain. Male WT (n = 18), female WT (n = 23), male Lcn2 KO (n = 24) and female Lcn2KO (n = 20) mice were fed on regular chow diet for 1 year. Average body weight of WT male and female (A), and Lcn2KO male and female (B) mice. Average body weight of WT versus Lcn2KO male (C) and female (D). P value < 0.05 considered as significant. Nonparametric Two-tailed t-test (Mann–Witney) was performed to compare the statistical significance.