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. 2020 Sep 3;10:14589. doi: 10.1038/s41598-020-71249-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lcn2 deficient female mice exhibited age-related weight gain and visceral fat deposition. One-year old female Lcn2 KO mice and WT mice weighing more than 25 g mice on regular chow diet. (A,C) Representative photographs of Lcn2KO and WT mice with weight gain and visceral fat (VF) accumulation. (B) Average of body weight of Lcn2KO (n = 8) and WT (n = 6) mice. (D) Average weight of visceral fat from Lcn2KO and WT mice. P value < 0.05 considered as significant. Nonparametric Two-tailed t-test (Mann-Witney) were performed to compare the statistical significance.