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. 2020 Sep 3;10:14606. doi: 10.1038/s41598-020-71762-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Placental gene expression profiles of SD and WIS rats. a Cytokine and chemokine gene expression measured by RT-qPCR. Values are the mean 2^−ΔCq ± SD determined by the comparative Cq method⁴⁰ using Actb as the reference gene (n = 6). ^#Indicates genes that were not detected by RT-qPCR in SD rats. (b) The mean fold response of the same cytokine and chemokine genes shown in (a). Values were generated by dividing the mean 2^−ΔCq (n = 6) of the infected group by the mean 2^−ΔCq (n = 6) of the corresponding control group. *Indicates the 2^−ΔCq values that were significantly affected by infection in panel (a) based on student’s t test. (c) Expression of genes involved in placental morphogenesis measured by RT-qPCR. Values are the mean 2^−ΔCq ± SD determined by the comparative Cq method⁴⁰ with the corresponding Actb used as the reference gene (n = 6). (d) The mean fold response of the same placental genes shown in panel c. *Indicates the 2^−ΔCq values that were significantly affected by infection in panel c based on Student’s t test. Composite image was created with Adobe Photoshop 2020 v. 21.1.3 (www.adobe.com).