Figure 2.

Knockdown of NFYA inhibits ccRCC cell proliferation. A. The expression of NFYA was measured in 293T, A498, ACHN, 786-O, Caki-1 and 769-P cells using western blot. B. The efficiency of NFYA knockdown in A498, ACHN, 786-O and 769-P cells by two si-RNAs was verified by western blot. C. Control or NFYA si-RNAs were transfected into ccRCC cells and cell viability was evaluated with MTT assays. Results were presented as the mean ± standard deviation (SD). D. The effect of NFYA knockdown on colony formation was measured in ccRCC cells. The clones were dissolved with glacial acetic acid and the absorbance was measured at 570 nm. The histogram shows the mean ± standard deviation (SD). **P < 0.01.