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TGF-ß1 inhibition or HIF1A antibody treatment induces the expression of angiogenic factors and hydroxyproline. Several angiogenic factors amplified by EGLN2/3 and TGF-ß signaling were suppressed by incubation with the HIF1A antibody (A and B). On the other hand, the effects of TGF-ß were suppressed by LY364947 (A and B). Moreover, hydroxyproline concentrations in the culture supernatants were not suppressed by LY364947; however, they were suppressed by the HIF1A antibody (C).
