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. 2020 Aug 1;10(8):2428–2445.

Figure 4.

Figure 4

DHX9 Interacts with SPOP and depends on the SBC motif. (A and B) IB analysis of WCLs and anti-Flag co-IPs (A) or anti-HA co-IPs (B) derived from 293T cells transfected with the indicated constructs. (C and D) IB analysis of WCLs and anti-Flag co-IPs (C) or anti-HA co-IPs (D) derived from JAR and JEG3 cells transfected with the indicated constructs. (E) IB analysis of WCLs and anti-DHX9 co-IPs derived from JAR cells and JEG3 cells. (F) Schematic diagram showing the specific amino acid of the SBC motif and the position of the SBC motif contained in DHX9. (G) IB analysis of WCLs and anti-Flag co-IPs derived from 293T cells transfected with the indicated constructs. Rabbit IgG was used as a negative control for co-IPs. Cells were treated with 10 μg/ml MG132 for 10 h before harvesting. HA-DHX9-ΔSBC, HA-DHX9 with SBC motif deleted.