Figure 6.

SPOP negatively regulates DHX9-mediated invasion and migration of JAR cells. A. Real-time PCR analysis of DHX9 mRNA levels in JAR cells transfected with sh-RNA plasmid. B and C. IB analysis of DHX9 protein levels derived in JAR cells transfected with the indicated constructs. D. Representative images of migrated and invaded JAR cells transfected with the indicated constructs in migration assay and invasion assay. E. Quantification analysis of migrated JAR cells and invaded JAR cells. F and G. IB analysis of DHX9, E-cadherin, Cytokeratin 8, N-cadherin and vimentin levels derived from JAR cells transfected with the indicated constructs. Data are shown as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t-test.