Figure 7.

Assessment of ovatodiolide as an inhibitor of β-catenin. A. AutoDock Vina Computational docking model for ovatodiolide and β-catenin. The highest binding affinity scenario is depicted. The amino acid residues (Asn516, Ser473, and Lys508) participate in binding with ovatodiolide are shown. B. The top ten different binding modes are ranked by the free energy (binding affinity). C. The chemical structure of ovatodiolide depicts three potential H-bond acceptors for reinforcing its binding with β-catenin. D. TCF assay. Ovatodiolide treatment (5 µM) significantly reduced β-catenin activity, as determined by the reduced luciferase activity of the TOP/FOP flash system.