Fig. 7. Effect of mol-5 on STAT1 activation and ISG expression.

THP-1 cells were infected with DENV2 (10¹ TCID₅₀) and further incubated with mol-5 (2.5, 5, or 10 μM) in maintenance medium after washing with virus-free medium. a The protein levels of p-STAT1 (Tyr701), total STAT1, E protein and actin were determined at 48 hpi. b After THP-1 cells were incubated with IFN-β (50 ng/mL) or mol-5 (10 μM), the p-STAT1 (Tyr701), total STAT1 and actin levels were determined at 48 hpi. c The mRNA levels of ISG15, ISG54, IFITM1, and IFITM3 were detected by qRT-PCR as indicated. d The expression levels of IL-8, IL-1β, and TNF-α were quantified using qRT-PCR. DMSO was used as a solvent control. Each data point represents the mean ± S.D. of an average of three separate trials. *** indicates P < 0.001.