Fig. 6.

Intracellular application of cocaine did not reduce α₃β₄-nAChR function. a After the establishment of whole-cell patch-clamp recording, 30 µM cocaine was perfused into the recorded cell over 20 min, while whole-cell inward currents mediated by α₃β₄ nAChRs to 20 µM nicotine were recorded in the absence or the presence of cocaine coapplied with nicotine extracellularly through the perfusion system. b Bar graph summarizing the effects of cocaine supplied under the conditions specified in a on peak current responses (normalized to the response to 20 µM nicotine alone), **P < 0.01 (baseline vs. cocaine 1.5 µM exposure and nicotine washout vs. cocaine 30 µM exposure)