Fig. 7.

Intracellular application of GDP-βS does not affect cocaine-mediated inhibition of α₃β₄-nAChR function. a Typical whole-cell inward current responses to 20 µM nicotine alone (horizontal black bar) or under the condition of nicotine plus 1.5 µM cocaine coapplication (horizontal open bar) were recorded after 600 µM GDP-βS was loaded into the recorded cell during the 20 min after conversion to the whole-cell recording mode. b Bar graph summarizing the effects of 1.5 µM cocaine on responses to 20 µM nicotine (peak currents normalized to those induced by 20 µM nicotine alone), ***P < 0.001 (baseline vs. cocaine exposure)