Fig. 6.

TLR4 deficiency ameliorated the activation of MPTP-induced NF-κB and the NLRP3 inflammasome signaling pathways. a Priming and subsequent activation of NLRP3 inflammasome. b–g The protein levels of NLRP3, ASC, caspase-1 (pro- and p20), IL-1β, and p-NF-κB/65 were analyzed by Western blotting. β-Actin protein levels served as loading controls. The data are expressed as the mean ± SD (n = 3). *P < 0.05, **P < 0.01 vs. control group of WT mice; ^#P<0.05, ^##P < 0.01 vs. MPTP-treated WT mice